analyzer software Search Results


tc20 tm data analyzer software  (Bio-Rad)
99
Bio-Rad tc20 tm data analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Tc20 Tm Data Analyzer Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tc20 tm data analyzer software - by Bioz Stars, 2026-05
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thermal analysis gas station  (Revvity)
90
Revvity thermal analysis gas station
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Thermal Analysis Gas Station, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pyris  (Revvity)
91
Revvity pyris
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Pyris, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pyris - by Bioz Stars, 2026-05
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acquire and analysis software  (Physiologic Instruments)
90
Physiologic Instruments acquire and analysis software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Acquire And Analysis Software, supplied by Physiologic Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brainvision analyzer  (brain products gmbh)
90
brain products gmbh brainvision analyzer
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Brainvision Analyzer, supplied by brain products gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiwell analyzer software  (Mcs GmbH)
90
Mcs GmbH multiwell analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Multiwell Analyzer Software, supplied by Mcs GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analyzer software  (Ionoptika Ltd)
90
Ionoptika Ltd analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Analyzer Software, supplied by Ionoptika Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analyzer software - by Bioz Stars, 2026-05
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bz-x analyzer software bz-h4a  (KEYENCE)
90
KEYENCE bz-x analyzer software bz-h4a
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Bz X Analyzer Software Bz H4a, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aida image analyzer v.422  (Raytest GmbH)
90
Raytest GmbH aida image analyzer v.422
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Aida Image Analyzer V.422, supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automated edge-detection brachial analyzer software  (Medical Imaging Applications LLC)
90
Medical Imaging Applications LLC automated edge-detection brachial analyzer software
Analysis of <t>brachial</t> diameter and velocity (using the <t>edge-detection</t> Brachial <t>Analyzer</t> <t>software)</t> of a representative subject immediately following cuff deflation.
Automated Edge Detection Brachial Analyzer Software, supplied by Medical Imaging Applications LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smart analyzer software  (SPECTRO Analytical)
90
SPECTRO Analytical smart analyzer software
Analysis of <t>brachial</t> diameter and velocity (using the <t>edge-detection</t> Brachial <t>Analyzer</t> <t>software)</t> of a representative subject immediately following cuff deflation.
Smart Analyzer Software, supplied by SPECTRO Analytical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nano analyze software  (TA Instruments)
90
TA Instruments nano analyze software
Analysis of <t>brachial</t> diameter and velocity (using the <t>edge-detection</t> Brachial <t>Analyzer</t> <t>software)</t> of a representative subject immediately following cuff deflation.
Nano Analyze Software, supplied by TA Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Journal: Cell reports

Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation

doi: 10.1016/j.celrep.2025.116758

Figure Lengend Snippet: (A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Article Snippet: TC20 TM Data Analyzer Software , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Flow Cytometry, Cell Characterization, Expressing, MANN-WHITNEY

Analysis of brachial diameter and velocity (using the edge-detection Brachial Analyzer software) of a representative subject immediately following cuff deflation.

Journal: Cardiovascular Ultrasound

Article Title: Normalization of flow-mediated dilation to shear stress area under the curve eliminates the impact of variable hyperemic stimulus

doi: 10.1186/1476-7120-6-44

Figure Lengend Snippet: Analysis of brachial diameter and velocity (using the edge-detection Brachial Analyzer software) of a representative subject immediately following cuff deflation.

Article Snippet: Off-line analyses of diameters and velocities were performed using an automated edge-detection Brachial Analyzer software (Medical Imaging Applications, LLC, Coralville, IA); briefly, the software allows the user to identify a region of interest (ROI) on the portion of the image where the vessel walls are most clear.

Techniques: Software